| 质粒名称: | 1136- Desmoplakin-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32227 |
| 存储实验室: | Kathleen Green |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Desmoplakin |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 8616 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | EGFP（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-Fwd |
| 3' 测序引物 | EGFP-N |
|
文献引用方法 |
|
| 材料和方法部分: | 1136- Desmoplakin-GFP was a gift from Kathleen Green (Addgene plasmid # 32227 ; http://n2t.net/addgene:32227 ; RRID:Addgene_32227) |
| 参考文献部分: | Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. J Cell Biol. 2005 Dec 19;171(6):1045-59. 10.1083/jcb.200510038 PubMed 16365169 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 1136- Desmoplakin-GFP 13661 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS 1136- Desmoplakin-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13661)
AUTHORS Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT,
Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew
TL, Green KJ
TITLE Desmoplakin assembly dynamics in four dimensions: multiple phases
differentially regulated by intermediate filaments and actin.
JOURNAL J Cell Biol. 2005 Dec 19;171(6):1045-59.
PUBMED 16365169
REFERENCE 2 (bases 1 to 13661)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13661
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 9608..9617
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 9614..10333
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(9659..9680)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(9920..9939)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 10267..10288
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 10456..10577
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10493..10512)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10547..10566
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(10584..11039)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10721..10742)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 10933..10952
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 11066..11170
/gene="bla"
/label=AmpR promoter
promoter 11172..11529
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(11195..11215)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 11380..11515
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 11442..11461
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 11564..12358
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(11618..11637)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 12228..12247
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(12546..12565)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 12590..12637
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 12966..13554
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 13455..13474
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgcg tcaacagccc
661 ctgctccttg ggcccctcca tgccatgccg taatctctcc cacccgacca acaccaacac
721 ccagctccga cgcagctcct ctgcgccctt gccgccctcc gagccacagc tttcctcccg
781 ctcctgcccc cggcccgtcg ccgtctccgc gctcgcagcg gcctcgggag ggcccaggta
841 gcgagcagcg acctcgcgag ccttccgcac tcccgcccgg ttccccggcc gtccgcctat
901 ccttggcccc ctccgctttc tccgcgccgg cccgcctcgc ttatgcctcg gcgctgagcc
961 gctctcccga ttgcccgccg acatgagctg caacggaggc tcccacccgc ggatcaacac
1021 tctgggccgc atgatccgcg ccgagtctgg cccggacctg cgctacgagg tgaccagcgg
1081 cggcgggggc accagcagga tgtactattc tcggcgcggc gtgatcaccg accagaactc
1141 ggacggctac tgtcaaaccg gcacgatgtc caggcaccag aaccagaaca ccatccagga
1201 gctgctgcag aactgctccg actgcttgat gcgagcagag ctcatcgtgc agcctgaatt
1261 gaagtatgga gatggaatac aactgactcg gagtcgagaa ttggatgagt gttttgccca
1321 ggccaatgac caaatggaaa tcctcgacag cttgatcaga gagatgcggc agatgggcca
1381 gccctgtgat gcttaccaga aaaggcttct tcagctccaa gagcaaatgc gagcccttta
1441 taaagccatc agtgtccctc gagtccgcag ggccagctcc aagggtggtg gaggctacac
1501 ttgtcagagt ggctctggct gggatgagtt caccaaacat gtcaccagtg aatgtttggg
1561 gtggatgagg cagcaaaggg cggagatgga catggtggcc tggggtgtgg acctggcctc
1621 agtggagcag cacattaaca gccaccgggg catccacaac tccatcggcg actatcgctg
1681 gcagctggac aaaatcaaag ccgacctgcg cgagaaatct gcgatctacc agttggagga
1741 ggagtatgaa aacctgctga aagcgtcctt tgagaggatg gatcacctgc gacagctgca
1801 gaacatcatt caggccacgt ccagggagat catgtggatc aatgactgcg aggaggagga
1861 gctgctgtac gactggagcg acaagaacac caacatcgct cagaaacagg aggccttctc
1921 catacgcatg agtcaactgg aagttaaaga aaaagagctc aataagctga aacaagaaag
1981 tgaccaactt gtcctcaatc agcatccagc ttcagacaaa attgaggcct atatggacac
2041 tctgcagacg cagtggagtt ggattcttca gatcaccaag tgcattgatg ttcatctgaa
2101 agaaaatgct gcctactttc agttttttga agaggcgcag tctactgaag catacctgaa
2161 ggggctccag gactccatca ggaagaagta cccctgcgac aagaacatgc ccctgcagca
2221 cctgctggaa cagatcaagg agctggagaa agaacgagag aaaatccttg aatacaagcg
2281 tcaggtgcag aacttggtaa acaagtctaa gaagattgta cagctgaagc ctcgtaaccc
2341 agactacaga agcaataaac ccattattct cagagctctc tgtgactaca aacaagatca
2401 gaaaatcgtg cataaggggg atgagtgtat cctgaaggac aacaacgagc gcagcaagtg
2461 gtacgtgacg ggcccgggag gcgttgacat gcttgttccc tctgtggggc tgatcatccc
2521 tcctccgaac ccactggccg tggacctctc ttgcaagatt gagcagtact acgaagccat
2581 cttggctctg tggaaccagc tctacatcaa catgaagagc ctggtgtcct ggcactactg
2641 catgattgac atagagaaga tcagggccat gacaatcgcc aagctgaaaa caatgcggca
2701 ggaagattac atgaagacga tagccgacct tgagttacat taccaagagt tcatcagaaa
2761 tagccaaggc tcagagatgt ttggagatga tgacaagcgg aaaatacagt ctcagttcac
2821 cgatgcccag aagcattacc agaccctggt cattcagctc cctggctatc cccagcacca
2881 gacagtgacc acaactgaaa tcactcatca tggaacctgc caagatgtca accataataa
2941 agtaattgaa accaacagag aaaatgacaa gcaagaaaca tggatgctga tggagctgca
3001 gaagattcgc aggcagatag agcactgcga gggcaggatg actctcaaaa acctccctct
3061 agcagaccag gggtcttctc accacatcac agtgaaaatt aacgagctta agagtgtgca
3121 gaatgattca caagcaattg ctgaggttct caaccagctt aaagatatgc ttgccaactt
3181 cagaggttct gaaaagtact gctatttaca gaatgaagta tttggactat ttcagaaact
3241 ggaaaatatc aatggtgtta cagatggcta cttaaatagc ttatgcacag taagggcact
3301 gctccaggct attctccaaa cagaagacat gttaaaggtt tatgaagcca ggctcactga
3361 ggaggaaact gtctgcctgg acctggataa agtggaagct taccgctgtg gactgaagaa
3421 aataaaaaat gacttgaact tgaagaagtc gttgttggcc actatgaaga cagaactaca
3481 gaaagcccag cagatccact ctcagacttc acagcagtat ccactttatg atctggactt
3541 gggcaagttc ggtgaaaaag tcacacagct gacagaccgc tggcaaagga tagataaaca
3601 gatcgacttt agattatggg acctggagaa acaaatcaag caattgagga attatcgtga
3661 taactatcag gctttctgca agtggctcta tgatgctaaa cgccgccagg attccttaga
3721 atccatgaaa tttggagatt ccaacacagt catgcggttt ttgaatgagc agaagaactt
3781 gcacagtgaa atatctggca aacgagacaa atcagaggaa gtacaaaaaa ttgctgaact
3841 ttgcgccaat tcaattaagg attatgagct ccagctggcc tcatacacct caggactgga
3901 aactctgctg aacataccta tcaagaggac catgattcag tccccttctg gggtgattct
3961 gcaagaggct gcagatgttc atgctcggta cattgaacta cttacaagat ctggagacta
4021 ttacaggttc ttaagtgaga tgctgaagag tttggaagat ctgaagctga aaaataccaa
4081 gatcgaagtt ttggaagagg agctcagact ggcccgagat gccaactcgg aaaactgtaa
4141 taagaacaaa ttcctggatc agaacctgca gaaataccag gcagagtgtt cccagttcaa
4201 agcgaagctt gcgagcctgg aggagctgaa gagacaggct gagctggatg ggaagtcggc
4261 taagcaaaat ctagacaagt gctacggcca aataaaagaa ctcaatgaga agatcacccg
4321 actgacttat gagattgaag atgaaaagag aagaagaaaa tctgtggaag acagatttga
4381 ccaacagaag aatgactatg accaactgca gaaagcaagg caatgtgaaa aggagaacct
4441 tggttggcag aaattagagt ctgagaaagc catcaaggag aaggagtacg agattgaaag
4501 gttgagggtt ctactgcagg aagaaggcac ccggaagaga gaatatgaaa atgagctggc
4561 aaaggtaaga aaccactata atgaggagat gagtaattta aggaacaagt atgaaacaga
4621 gattaacatt acgaagacca ccatcaagga gatatccatg caaaaagagg atgattccaa
4681 aaatcttaga aaccagcttg atagactttc aagggaaaat cgagatctga aggatgaaat
4741 tgtcaggctc aatgacagca tcttgcaggc cactgagcag cgaaggcgag ctgaagaaaa
4801 cgcccttcag caaaaggcct gtggctctga gataatgcag aagaagcagc atctggagat
4861 agaactgaag caggtcatgc agcagcgctc tgaggacaat gcccggcaca agcagtccct
4921 ggaggaggct gccaagacca ttcaggacaa aaataaggag atcgagagac tcaaagctga
4981 gtttcaggag gaggccaagc gccgctggga atatgaaaat gaactgagta aggtaagaaa
5041 caattatgat gaggagatca ttagcttaaa aaatcagttt gagaccgaga tcaacatcac
5101 caagaccacc atccaccagc tcaccatgca gaaggaagag gataccagtg gctaccgggc
5161 tcagatagac aatctcaccc gagaaaacag gagcttatct gaagaaataa agaggctgaa
5221 gaacactcta acccagacca cagagaatct caggagggtg gaagaagaca tccaacagca
5281 aaaggccact ggctctgagg tgtctcagag gaaacagcag ctggaggttg agctgagaca
5341 agtcactcag atgcgaacag aggagagcgt aagatataag caatctcttg atgatgctgc
5401 caaaaccatc caggataaaa acaaggagat agaaaggtta aaacaactga tcgacaaaga
5461 aacaaatgac cggaaatgcc tggaagatga aaacgcgaga ttacaaaggg tccagtatga
5521 cctgcagaaa gcaaacagta gtgcgacgga gacaataaac aaactgaagg ttcaggagca
5581 agaactgaca cgcctgagga tcgactatga aagggtttcc caggagagga ctgtgaagga
5641 ccaggatatc acgcggttcc agaactctct gaaagagctg cagctgcaga agcagaaggt
5701 ggaagaggag ctgaatcggc tgaagaggac cgcgtcagaa gactcctgca agaggaagaa
5761 gctggaggaa gagctggaag gcatgaggag gtcgctgaag gagcaagcca tcaaaatcac
5821 caacctgacc cagcagctgg agcaggcatc cattgttaag aagaggagtg aggatgacct
5881 ccggcagcag agggacgtgc tggatggcca cctgagggaa aagcagagga cccaggaaga
5941 gctgaggagg ctctcttctg aggtcgaggc cctgaggcgg cagttactcc aggaacagga
6001 aagtgtcaaa caagctcact tgaggaatga gcatttccag aaggcgatag aagataaaag
6061 cagaagctta aatgaaagca aaatagaaat tgagaggctg cagtctctca cagagaacct
6121 gaccaaggag cacttgatgt tagaagaaga actgcggaac ctgaggctgg agtacgatga
6181 cctgaggaga ggacgaagcg aagcggacag tgataaaaat gcaaccatct tggaactaag
6241 gagccagctg cagatcagca acaaccggac cctggaactg caggggctga ttaatgattt
6301 acagagagag agggaaaatt tgagacagga aattgagaaa ttccaaaagc aggctttaga
6361 ggcatctaat aggattcagg aatcaaagaa tcagtgtact caggtggtac aggaaagaga
6421 gagccttctg gtgaaaatca aagtcctgga gcaagacaag gcaaggctgc agaggctgga
6481 ggatgagctg aatcgtgcaa aatcaactct agaggcagaa accagggtga aacagcgcct
6541 ggagtgtgag aaacagcaaa ttcagaatga cctgaatcag tggaagactc aatattcccg
6601 caaggaggag gctattagga agatagaatc ggaaagagaa aagagtgaga gagagaagaa
6661 cagtcttagg agtgagatcg aaagactcca agcagagatc aagagaattg aagagaggtg
6721 caggcgtaag ctggaggatt ctaccaggga gacacagtca cagttagaaa cagaacgctc
6781 ccgatatcag agggagattg ataaactcag acagcgccca tatgggtccc atcgagagac
6841 ccagactgag tgtgagtgga ccgttgacac ctccaagctg gtgtttgatg ggctgaggaa
6901 gaaggtgaca gcaatgcagc tctatgagtg tcagctgatc gacaaaacaa ccttggacaa
6961 actattgaag gggaagaagt cagtggaaga agttgcttct gaaatccagc cattccttcg
7021 gggtgcagga tctatcgctg gagcatctgc ttctcctaag gaaaaatact ctttggtaga
7081 ggccaagaga aagaaattaa tcagcccaga atccacagtc atgcttctgg aggcccaggc
7141 agctacaggt ggtataattg atccccatcg gaatgagaag ctgactgtcg acagtgccat
7201 agctcgggac ctcattgact tcgatgaccg tcagcagata tatgcagcag aaaaagctat
7261 cactggtttt gatgatccat tttcaggcaa gacagtatct gtttcagaag ccatcaagaa
7321 aaatttgatt gatagagaaa ccggaatgcg cctgctggaa gcccagattg cttcaggggg
7381 tgtagtagac cctgtgaaca gtgtcttttt gccaaaagat gtcgccttgg cccgggggct
7441 gattgataga gatttgtatc gatccctgaa tgatccccga gatagtcaga aaaactttgt
7501 ggatccagtc accaaaaaga aggtcagtta cgtgcagctg aaggaacggt gcagaatcga
7561 accacatact ggtctgctct tgctttcagt acagaagaga agcatgtcct tccaaggaat
7621 cagacaacct gtgaccgtca ctgagctagt agattctggt atattgagac cgtccactgt
7681 caatgaactg gaatctggtc agatttctta tgacgaggtt ggtgagagaa ttaaggactt
7741 cctccagggt tcaagctgca tagcaggcat atacaatgag accacaaaac agaagcttgg
7801 catttatgag gccatgaaaa ttggcttagt ccgacctggt actgctctgg agttgctgga
7861 agcccaagca gctactggct ttatagtgga tcctgttagc aacttgaggt taccagtgga
7921 ggaagcctac aagagaggtc tggtgggcat tgagttcaaa gagaagctcc tgtctgcaga
7981 acgagctgtc actgggtata atgatcctga aacaggaaac atcatctctt tgttccaagc
8041 catgaataag gaactcatcg aaaagggcca cggtattcgc ttattagaag cacagatcgc
8101 aaccgggggg atcattgacc caaaggagag ccatcgttta ccagttgaca tagcatataa
8161 gaggggctat ttcaatgagg aactcagtga gattctctca gatccaagtg atgataccaa
8221 aggatttttt gaccccaaca ctgaagaaaa tcttacctat ctgcaactaa aagaaagatg
8281 cattaaggat gaggaaacag ggctctgtct tctgcctctg aaagaaaaga agaaacaggt
8341 gcagacatca caaaagaata ccctcaggaa gcgtagagtg gtcatagttg acccagaaac
8401 caataaagaa atgtctgttc aggaggccta caagaagggc ctaattgatt atgaaacctt
8461 caaagaactg tgtgagcagg aatgtgaatg ggaagaaata accatcacgg gatcagatgg
8521 ctccaccagg gtggtcctgg tagatagaaa gacaggcagt cagtatgata ttcaagatgc
8581 tattgacaag ggccttgttg acaggaagtt ctttgatcag taccgatccg gcagcctcag
8641 cctcactcaa tttgctgaca tgatctcctt gaaaaatggt gtcggcacca gcagcagcat
8701 gggcagtggt gtcagcgatg atgtttttag cagctcccga catgaatcag taagtaagat
8761 ttccaccata tccagcgtca ggaatttaac cataaggagc agctcttttt cagacaccct
8821 ggaagaatcg agccccattg cagccatctt tgacacagaa aacctggaga aaatctccat
8881 tacagaaggt atagagcggg gcatcgttga cagcatcacg ggtcagaggc ttctggaggc
8941 tcaggcctgc acaggtggca tcatccaccc aaccacgggc cagaagctgt cacttcagga
9001 cgcagtctcc cagggtgtga ttgaccaaga catggccacc aggctgaagc ctgctcagaa
9061 agccttcata ggcttcgagg gtgtgaaggg aaagaagaag atgtcagcag cagaggcagt
9121 gaaagaaaaa tggctcccgt atgaggctgg ccagcgcttc ctggagttcc agtacctcac
9181 gggaggtctt gttgacccgg aagtgcatgg gaggataagc accgaagaag ccatccggaa
9241 ggggttcata gatggccgcg ccgcacagag gctgcaagac accagcagct atgccaaaat
9301 cctgacctgc cccaaaacca aattaaaaat atcctataag gatgccataa atcgctccat
9361 ggtagaagat atcactgggc tgcgccttct ggaagccgcc tccgtgtcgt ccaagggctt
9421 acccagccct tacaacatgt cttcggctcc ggggtcccgc tccggctccc gctcgggatc
9481 tcgctccgga tctcgctccg ggtcccgcag tgggtcccgg agaggaagct ttgacgccac
9541 agggaattct tcctactctt attcctactc atttagcagt agttctattg ggcacgatcc
9601 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
9661 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
9721 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
9781 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
9841 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
9901 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
9961 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
10021 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
10081 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
10141 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
10201 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
10261 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
10321 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
10381 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
10441 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
10501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
10561 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
10621 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
10681 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
10741 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
10801 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
10861 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
10921 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
10981 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
11041 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
11101 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
11161 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
11221 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
11281 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
11341 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
11401 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
11461 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
11521 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
11581 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
11641 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
11701 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
11761 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
11821 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
11881 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
11941 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
12001 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
12061 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
12121 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
12181 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
12241 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
12301 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
12361 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
12421 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
12481 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
12541 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
12601 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
12661 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
12721 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
12781 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
12841 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
12901 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
12961 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
13021 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
13081 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
13141 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
13201 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
13261 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
13321 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
13381 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
13441 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
13501 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
13561 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
13621 cctgcgttat cccctgattc tgtggataac cgtattaccg c
//
